boc gln  (Chem Impex International)

Journal: eLife

Article Title: Metabolic clogging of mannose triggers dNTP loss and genomic instability in human cancer cells

doi: 10.7554/eLife.83870

Figure Lengend Snippet:

Article Snippet: Approximately 8 × 10 6 cells were washed two times with 5 mL of 5% (w/v) mannitol (133-00845, FUJIFILM Wako) and scraped in 1.3 mL of methanol (138-14521, FUJIFILM Wako) that was spiked with 10 μM external standards (Human Metabolome Technologies).

Techniques: Retroviral, Recombinant, Drug discovery, Plasmid Preparation, Expressing, Sequencing, CRISPR, Lactate Dehydrogenase Assay, Cloning, Protease Inhibitor, Software

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: Expression levels of OsJAZ1 under various abiotic stresses and phytohormone treatments. (A–F) Seedlings at the four-leaf stage were subjected to treatment with drought stress, NaCl (200 mmol/L), cold (4°C), ABA (100 μmol/L), MeJA (100 μmol/L), and SA (100 μmol/L), respectively. Error bars represent the SE of three replicates. Significance was determined by Student’s t -test ( ∗∗∗ P < 0.001, ∗∗ P < 0.01, ∗ P < 0.05).

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Expressing

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: Phenotypes of the OsJAZ1 -OE plants and the jaz1 T-DNA insertion mutant plants under drought stress treatment at the seedling stage. (A) Schematic diagram of the OsJAZ1 gene and 4A-00845 T-DNA insertion mutant. UTR, ORF, and introns are indicated in the black box, white box, and line, respectively. (B) Expression levels of OsJAZ1 in the 4A-00845 homozygous line and Dongjin. (C) The expression levels of OsJAZ1 in the OE lines and WT ZH11. (D,E) Drought tolerance testing of jaz1 T-DNA mutant plants and over-expression plants. (D) The jaz1 mutant lines showed increased drought resistance. (E) The OsJAZ1 -OE plants were more sensitive to drought stress treatment compared to WT ZH11. (F,G) The survival rates of the transgenic and WT plants after recovery from drought stress treatment for 7 days. Mut-1 and mut-2 were heterozygous mutants segregated from 4A-00845. Error bars represent the SE of three replicates ( ∗∗∗ P < 0.005, Student’s t -test).

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Mutagenesis, Expressing, Over Expression, Transgenic Assay

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: Drought resistance testing of the OsJAZ1 -OE and mutant lines at the reproductive stage. (A) The OsJAZ1 -OE lines and the WT plants grown in barrels at the booting stage were subjected to drought stress treatment for 7 days with the soil moisture content maintained at 15%. The degree of leaf rolling is indicated by the PAR (B) and the relative water content (RWC) (C) of the OsJAZ1 -OE lines under normal and drought stress conditions. PAR = plant perimeter/plant projected area. (D) The jaz1 mutant lines showed increased drought resistance. PAR (E) and RWC (F) of the jaz1 mutant lines and Dongjin under normal and drought stress conditions. Error bars represent the SE of three replicates ( ∗∗ P < 0.01, ∗ P < 0.05, Student’s t -test).

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Mutagenesis

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: ABA and MeJA sensitivity assay of the OsJAZ1 -OE and T-DNA mutant plants. (A,B) The OE lines and the mutant lines were treated with 3 μM ABA and 10 μM MeJA, under normal conditions (no addition of ABA or MeJA) as a control. (C–F) Shoot and root length of the seedlings were measured to estimate the sensitivity of the WT and transgenic plants. Error bars represent the SE of three replicates ( ∗∗∗ P < 0.005, ∗∗ P < 0.01, ∗ P < 0.05, Student’s t -test).

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Sensitive Assay, Mutagenesis, Control, Transgenic Assay

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: OsJAZ1 subcellular localization. (A,B) Confocal microscopy of OsJAZ1-YFP localization in rice protoplasts under normal conditions and 100 μM MeJA treatment, respectively. (C) 35S: YFP (PM999-YFP) was transformed into rice protoplasts as a control. GHD7 was used as a nucleus marker. Bars = 20 μm.

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Confocal Microscopy, Transformation Assay, Control, Marker

Journal: Frontiers in Plant Science

Article Title: OsJAZ1 Attenuates Drought Resistance by Regulating JA and ABA Signaling in Rice

doi: 10.3389/fpls.2017.02108

Figure Lengend Snippet: Transcriptome profiling of OsJAZ1 -OE plants. (A) The expression patterns of the DEGs under normal (–N) and drought stress conditions (–D). Color scale is in log 2 of the fold change. (B) Number of specific and common drought-responsive DEGs in the WT and OE plants. (C) KEGG classification of the specific drought-responsive DEGs (Group I and Group II) in the WT and OsJAZ1 -OE plants. (D) Confirmation of several genes involved in the ABA and JA pathways and stress resistance showing lower drought-induced levels in the OsJAZ1- OE plants than in WT ZH11. Error bars represent the SE of three replicates.

Article Snippet: The OsJAZ1 mutant line 4A-00845 in the background of the rice variety Dongjin ( Oryza sativa L. ssp. japonica ) was obtained from the POSTECH RISD .

Techniques: Expressing